This application note presents a comparative study of enzymatically manufactured doggybone DNA™ (dbDNA™) versus traditional plasmid DNA (pDNA) for lentiviral vector (LVV) production, using the FuGENE® 4K transfection reagent. The study evaluates transient transfection of third‑generation lentiviral systems in HEK293T cells, assessing performance across both adherent and suspension culture processes relevant to scalable LVV manufacturing.

The document details experimental results showing that dbDNA, when used with FuGENE® 4K, delivers higher infectious lentiviral titres than pDNA across multiple transfection conditions. Up to three‑fold higher titres were achieved in adherent cultures, with 2.4‑fold higher titres in suspension systems, alongside improved performance at lower DNA inputs. The findings also highlight cost‑of‑goods advantages and the suitability of dbDNA’s cell‑free, fermentation‑free manufacturing process for research and GMP‑aligned LVV production.

Download this application note to:

• Review comparative data on dbDNA vs pDNA performance in lentiviral vector production
• Learn how transfection parameters impact LVV yield in adherent and suspension HEK293T systems
• Understand the yield, scalability, and cost implications of using dbDNA with FuGENE® 4K