Advancements in synthetic DNA technologies are transforming the landscape of vaccine and therapeutic development. Plasmid DNA (pDNA) is widely used as a template for in vitro transcription (IVT) in RNA manufacturing; however, conventional pDNA production can limit manufacturing speed, scalability, and flexibility. Enzymatically produced z-dbDNA™ is a closed, linear, double-stranded DNA molecule that lacks bacterial backbone sequences and represents an alternative DNA template for IVT applications. Here, we evaluate z-dbDNA templates and compare their performance with traditional linearised pDNA.

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